Patankar Fertility Solutions has the state-of-the-art ART laboratory with the specific areas dedicated to carry out the various procedures involved in the ART (Artificial Reproductive Techniques).
Entire floor is exclusively dedicated to carry out different ART Procedures. This avoids unnecessary crowd, helping to maintain the privacy as well as sterility.
Quality Control And Quality Assurance.
Our ART Lab is well equipped with the most advanced and latest version of equipments necessary for all ART procedures. Proper functioning of the equipments and
Maintenance of laboratory culture conditions are most importance to get the best results and we, at Patankar fertility solutions take every effort to do so by doing not only
In house maintenance on daily basis but also periodic maintenance from the certified
All the procedures are carried out as per the ICMR Guidelines.
ART Lab has following sections
Non Sterile zone
- Counseling Cell
- Patients waiting area
- Collection Room
- IUI Room
- Cryopreservation Room
- Andrology Laboratory
- Operation Theatre
- Embryology Laboratory
In this Counseling is done to all the patients undergoing the treatments of infertility. The patient education material is also available.
There is a separate room with adequate privacy for the collection of the semen. Semen needs to be collected for the purpose of the semen analysis, semen processing for IUI as well as IVF and ICSI and also for the cryopreservation i.e. freezing. For patients who are not able to give semen sample by masturbation, vibrator facility is also available.
The Andrology lab is utilized to carry out all the procedures related to the semenology.
The lab is equipped with following Equipments
1) Computer Assisted Semen Analysis (CASA)
This is the machine which analyse the semen parameters through computerized Programmers.
This works beyond human eyes with more details and precision.
It analyses motility (exact speed), exact
dimension of the sperm and differentiates
between alive and dead sperms.
Sperm DNA fragmentation by computerized programme
We, at Patankar fertility solutions Pvt Ltd have latest version of CASA.
2) Laminar Air Flow
It gives highly purified air and surface
maintained with temperature of 37 ℃ for
Handling semenological procedures.
3)CO 2 Incubators:- Hera cell 150i
It is used for the incubation of the sperm.
It provides physiological Conditions,
maintaining the temperature of 37 ℃
and CO2 at desire percentage.
This is an advanced type of centrifuge which is used to process the Semen for IUI, IVF-ICSI. It maintains the temperature of 37 ℃ through the Process. It is fully automatic machine which takes care of the sperms very well doing minimum harm.
Latest version of microscope will gives us good resolution and magnification which is helpful to analysethe semen parameters very well.
This operation theatre is dedicated only for the
Procedures related to ART i.e. oocyte Retrieval,
Embryo transfer,testicular biopsy, embryo reduction,
etc. Being utilized only for such procedures, the
Microorganism-free environment is maintained.
Embryology laboratory is the heart of any ART programme. To achieve maximum Success rate,
the culture conditions of the lab have to be maintained
properly and Consistently. The ART Lab is under
microbiological surveillance in which there is periodic
checking of the growth of contaminating micro-
organisms. We, at Patankar Fertility Solutions, are
proud to say that our heart is in a very healthy
condition and so is able to give the maximum
results. It is equipped with
1) Laminar Air Flow
All the handling of the gametes and the embryos is carried out inthe laminar flow
Which maintains the emission of the pure air, the surface is heated at 37 ℃, the
Microscope and the incubator are kept right inside the laminar flow. This provides the
Optimum conditions for the gametes and the embryos.
Incubators provide optimum culture conditions for the proper growth of the embryos. In our laboratory we have total 6(4 –CO2 incubators,2-Trigas )Incubators. This has few advantages example-load (embryos, gametes and media) is distributed separately so that exposure will be reduced to minimum as the frequent opening and closing of the incubators is avoided. This way gametes, embryos are cultured in optimum conditions undisturbed
CO 2 Incubators
These incubators give the same physiological concentration of CO2 and O2 outside the human body and maintain at 37 ℃ temperature
Advance and each chamber is separated from each other and controlledseparately . It provides minimum exposure to other compartments.
3) Micromanipulator with Intracytoplasmic Morphologically Selected sperm Injection(IMSI)
We have latest version of Olympus-Narashige Micromanipulator
Micromanipulator use to prefer ICSI(Intracytoplasmic Sperm Injection),Assisted Hatching, Embryo biopsy.Evaluation of gametes, embryos and Blastocysts
It is attached with IMSI which gives the best Evaluation of sperm for injection (example- Vacuole inside sperm) and all other organelles of spermatozoa
4) Air Purification Untis
Air quality is very important in ART Laboratory as it is important to mimic the conditions similar to the inside of human body. This is achieve with the help of positive pressure unit. Blowing the fresh particle free and VOC free air inside embryology lab through positive pressure module.
Inside the laboratory NASA technology Airocide unit is installed which gives sterile air for removal of volatile organic compound(VOC) example. Alcohols inside the laboratory Aero500 unit is Installed. It absorbs VOC and gives VOC free air. These contain filter (HEPA, KMno4,CODA)which plays important role in filtering the air and need to Change periodically
There are 2 insemination rooms for IUI with adequate privacy. The processed semen is Inseminated under all aseptic precautions and the patient can rest for a while.
In this room we preserve the sperms, the embryos and the eggs, too. We have the Planer, which is used for the slow freezing and the CMV kit which is used for the rapid cooling i.e. vitrfication. The gametes and the embryos are maintained in liquid nitrogen at -196°C preserving their reproductive potential for years together. We are consistently getting more than 35% results with our Frozen-thaw cycles.For this we have separate
cans for semen which capacity storing the semen of around
4000 patient’s Semenstorage at a time.
Separate cans for the storage of embryos.
Quarantine cans are also maintained
separately for specific cases.
USG is the most important tool in the management of the infertile couples.It is used in many different ways.
- Base line USG
- Follicular monitoring
- Other Uses of USG
- Ovum pick-up
- Embryo Transfer
Base line USG
Baseline USG helps in the diagnosis of various conditions like fibroids, uterine polyps, PCOD, various anomalies of the uterus like septate uterus, didelphus uterus .
It is also done for the AFC i.e. Antral Follicular Count so that the response to the injection can be predicted and the treatment protocol can be decided for the patients undergoing IVF/ICSI.
It is very important to know the response of the simple treatment like ovulation induction in cases of PCOD or very advanced treatment like COH (Controlled Ovarian Hyperstimulation) in IVF and ICSI. The response is judged by counting the number of follicles developed,to know if the ovulation has occurred or not, if not then the trigger is given with the help of a injection and also to know the thickness of the endometrial. In cases of IVF and ICSI cycles, the monitoring is done to know the response of the injections being given and adjusting the doses accordingly, to schedule the timing of the ovum pickup or oocyte retrieval and to know the endometrial thickness.
Doppler has limited but definite role in the infertility management.
It is also sometimes used in the treatment of IVF/ICSI or to diagnose ectopic pregnancies.
Other Uses of USG
Ultrasound is used for ovum pick up as well as embryo transfer in cases of IVF and ICSI.
We at Patankar Fertility Solutions have the best USG machines. Also USGs are performed throughout the day to suit the patients needs.
For the Ovum pick-up a needle is attached to the transvaginal probe which is then guided into graffian follicle under ultrasound guidance. After entering into the graffian follicle negative pressure is applied to the needle which aspirates the oocyte (egg) with the ultrasound guidance the procedure of ovum pick up has become extremely easy and takes only 15-20 minutes.
We at Patankar Fertility Solutions do all the embryo transfers under USG guidance as it helps in exact placement of embryos in the uterine cavity.